Preparation of the monoclonal antibody against human Bocavirus VP2 protein / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 63-65, 2012.
Article
in Zh
| WPRIM
| ID: wpr-246179
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To express and purify HBoV VP2 protein, and the monoclonal antibody against HBoV VP2 protein was prepared with hybridoma technique.</p><p><b>METHODS</b>The HBoV VP2 cloned into vector pET-30a was expressed in E. coil. After purified by immobilized metal affinity chromatography, the BALB/c mouse was immunized with purified protein as antigen. The positive hybridoma cells were screened with hybridoma technique and ELISA assay. Isotype and titer of the monoclonal antibody were detected.</p><p><b>RESULTS</b>The recombinant HBoV VP2 protein was expressed and purified, and then the monoclonal antibody was obtained with hybridoma technique. The titer of the IgG monoclonal antibody was up to 14 x 10(5).</p><p><b>CONCLUSION</b>Monoclonal antibody against recombinant HBoV VP2 protein was prepared and the antibody titer was high. This work may provide a new method in rapid diagnosis and study of HBoV.</p>
Full text:
1
Index:
WPRIM
Main subject:
Plasmids
/
Recombinant Proteins
/
Capsid Proteins
/
Allergy and Immunology
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Human bocavirus
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Genetics
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Hybridomas
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Mice, Inbred BALB C
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Antibodies, Monoclonal
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2012
Type:
Article