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Down-regulation of AnnexinⅡExpression in NB4 Cells Induced by all-trans Retinoic Acid and Its Mechanisms / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1008-1013, 2016.
Article in Zh | WPRIM | ID: wpr-246826
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore effect of all-trans retinoic acid(ATRA) on annexin Ⅱ expression in NB4 cells and to analyze the luciferase activity of annexinⅡ promoter in condition of ATRA-induced treatment.</p><p><b>METHODS</b>NB4 cells were cultured in vitro, the transcriptional or translational expression levels of Annexin Ⅱ in NB4 cells treated with 1 µmol/L ATRA at different time points were detected by RT-PCR or Western blot respectively. Annexin Ⅱ-promoter was constructed, the recombinant plasmids pGL4.15 -Annexin Ⅱ -promoter were transfected into NB4 cells with electroporation, and after being treated with 1 µmol/L ATRA for 24 hours the luciferase acttivity of Annexin Ⅱ promoter was determined by luciferase activity assay.</p><p><b>RESULTS</b>The transcriptional expression of Annexin Ⅱ was down-regulated after 48 h. The translation expression of Annexin Ⅱ was slowly weakened after 24 h, and it was seriously reduced after 48 h. Further, Luciferase activity of AnnexinⅡ promoter in NB4 cells treated with 1 µmol/L ATRA was down-regulated, and showed a decreased tendency at indicated time points.</p><p><b>CONCLUSION</b>All-trans retinoic acid can induce the down-regulation of AnnexinⅡ expression on the membrane of NB4 cells, and the activity of Annexin Ⅱpromoter is down-regulated too. This study provide a basis for further study of molecular mechanism.</p>
Subject(s)
Full text: 1 Index: WPRIM Main subject: Tretinoin / Down-Regulation / Cell Differentiation / Annexin A2 / Cell Line, Tumor Limits: Humans Language: Zh Journal: Journal of Experimental Hematology Year: 2016 Type: Article
Full text: 1 Index: WPRIM Main subject: Tretinoin / Down-Regulation / Cell Differentiation / Annexin A2 / Cell Line, Tumor Limits: Humans Language: Zh Journal: Journal of Experimental Hematology Year: 2016 Type: Article