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Combined effects of 50 Hz magnetic field and magnetic nanoparticles on the proliferation and apoptosis of PC12 cells / 生物医学与环境科学(英文)
Biomed. environ. sci ; Biomed. environ. sci;(12): 97-105, 2014.
Article in En | WPRIM | ID: wpr-247079
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the bioeffects of extremely low frequency (ELF) magnetic field (MF) (50 Hz, 400 μT) and magnetic nanoparticles (MNPs) via cytotoxicity and apoptosis assays on PC12 cells.</p><p><b>METHODS</b>MNPs modified by SiO₂ (MNP-SiO₂) were characterized by transmission electron microscopy (TEM), dynamic light scattering and hysteresis loop measurement. PC12 cells were administrated with MNP-SiO2 with or without MF exposure for 48 h. Cytotoxicity and apoptosis were evaluated with MTT assay and annexin V-FITC/PI staining, respectively. The morphology and uptake of MNP-SiO₂ were determined by TEM. MF simulation was performed by Ansoft Maxwell based on the finite element method.</p><p><b>RESULTS</b>MNP-SiO₂ were identified as ~20 nm (diameter) ferromagnetic particles. MNP-SiO₂ reduced cell viability in a dose-dependent manner. MF also reduced cell viability with increasing concentrations of MNP-SiO₂. MNP-SiO₂ alone did not cause apoptosis in PC12 cells; instead, the proportion of apoptotic cells increased significantly under MF exposure and increasing doses of MNP-SiO₂. MNP-SiO₂ could be ingested and then cause a slight change in cell morphology.</p><p><b>CONCLUSION</b>Combined exposure of MF and MNP-SiO₂ resulted in remarkable cytotoxicity and increased apoptosis in PC12 cells. The results suggested that MF exposure could strengthen the MF of MNPs, which may enhance the bioeffects of ELF MF.</p>
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Full text: 1 Index: WPRIM Main subject: PC12 Cells / Apoptosis / Silicon Dioxide / Microscopy, Electron, Transmission / Cell Proliferation / Toxicity / Magnetite Nanoparticles / Magnetic Fields Limits: Animals Language: En Journal: Biomed. environ. sci Year: 2014 Type: Article
Full text: 1 Index: WPRIM Main subject: PC12 Cells / Apoptosis / Silicon Dioxide / Microscopy, Electron, Transmission / Cell Proliferation / Toxicity / Magnetite Nanoparticles / Magnetic Fields Limits: Animals Language: En Journal: Biomed. environ. sci Year: 2014 Type: Article