Identification of a novel HLA allele B 4446 by sequencing-based typing / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 518-519, 2007.
Article
in Zh
| WPRIM
| ID: wpr-247279
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify a novel HLA allele in Chinese population.</p><p><b>METHODS</b>A new HLA-B allele was initially detected by an usual PCR-SSP and PCR-SSOP in routine typing HLA allele. Sequence-based typing (SBT) was used to identify and analysis the difference between the new allele and HLA-B 4409 allele.</p><p><b>RESULTS</b>The HLA-B exon 3 nucleotide sequence of the novel allele was different from all other known alleles. The allele had 3 nucleotides replaced of the closest matching B 4409 allele at nt538(G>C), nt539(A>T) and nt540 (C>G) in exon 3, resulting in an amino acid change from D(GAC) to L(CTG) at codon 180.</p><p><b>CONCLUSION</b>A novel HLA allele was confirmed by the SBT and it was officially designated as HLA-B 4446 by WHO Nomenclature Committee for Factors of the HLA System in September,2005.</p>
Full text:
1
Index:
WPRIM
Main subject:
Molecular Sequence Data
/
Base Sequence
/
HLA-B Antigens
/
Classification
/
Sequence Analysis, DNA
/
Alleles
/
Genetics
Type of study:
Prognostic_studies
Limits:
Humans
/
Male
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2007
Type:
Article