Mutation detection of parkin gene by denaturing high performance liquid chromatography / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 449-452, 2007.
Article
in Zh
| WPRIM
| ID: wpr-247295
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect parkin gene mutation of early-onset parkinsonism (EOP) by denaturing high performance liquid chromatography (DHPLC).</p><p><b>METHODS</b>The blood cell genomic DNA of 82 EOP patients was isolated. Exons of parkin gene were amplified by PCR. The PCR products were detected by DHPLC. The sample with abnormal peak shape was sequenced.</p><p><b>RESULTS</b>Three point mutations were identified in 82 EOP patients compared with 100 healthy controls. Mutations in intron include IVS1-39 G --> T and IVS9 +18 C --> T. The T1422C mutation was in coding region and resulted in 441 Cys --> Arg.</p><p><b>CONCLUSION</b>Three heterozygous mutations are found in sporadic EOP patients and genetic diagnosis of parkin gene by DHPLC is applicable in EOP patients.</p>
Full text:
1
Index:
WPRIM
Main subject:
Parkinson Disease
/
DNA Mutational Analysis
/
Base Sequence
/
Polymerase Chain Reaction
/
Chromatography, High Pressure Liquid
/
Ubiquitin-Protein Ligases
/
Diagnosis
/
Genetics
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Methods
/
Mutation
Type of study:
Diagnostic_studies
Limits:
Adult
/
Humans
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2007
Type:
Article