Overexpression of adiponectin prevents hepatocyte steatosis / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 213-216, 2010.
Article
in Zh
| WPRIM
| ID: wpr-247555
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of adiponectin on hepatocyte steatosis.</p><p><b>METHODS</b>L02 cells were transfected with pEGFP-N1-AdipoQ, a plasmid encoding pEGFP-adiponectin fusion protein, or pEGFP-N1. Lipid droplets in the hepatocytes were observed by oil red staining at 72 h. The contents of TG, FFA and glycerol in hepatocytes were measured.</p><p><b>RESULTS</b>Compared to cells transfected with pEGFP-N1-AdipoQ plasmid, much more lipid droplets were observed in cells transfected with pEGFP-N1 plasmid. TG, FFA and glycerol contents in L02 cells and L02/pEGFP-N1 cells were significantly higher than those in L02/pEGFP-N1-AdipoQ cells.</p><p><b>CONCLUSIONS</b>Overexpression of adiponectin prevent hepatocyte steatosis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Plasmids
/
Triglycerides
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Recombinant Fusion Proteins
/
Transfection
/
Cell Line
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Hepatocytes
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Cell Biology
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Adiponectin
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Fatty Acids, Nonesterified
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Fatty Liver
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2010
Type:
Article