Effects of silencing chloride intracellular channel 1 gene expression on the proliferation and invasion of mouse hepatocellular carcinoma cell lines / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 131-135, 2010.
Article
in Zh
| WPRIM
| ID: wpr-247578
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of silencing CLIC1 gene expression on the proliferation and invasion of Hca-F cells.</p><p><b>METHODS</b>The mouse CLIC1 cDNA sequence was retrieved from NCBI. Three shRNA sequences were designed and cloned into pGPU6/GFP/Neo plasmids. The plasmids were transfected into Hca-F cells with Lipofectamine 2000. Cell Counting-8 (CCK-8) kit and transwell chamber were used to study the effects of CLIC1 on the proliferation and invasion of Hca-F cells.</p><p><b>RESULTS</b>The pGPU6/GFP/Neo-shRNA-3 plasmid effectively repressed the expression of CLIC1 mRNA. Inhibition of CLIC1 gene expression led to decreased cell proliferation and reduced invasion.</p><p><b>CONCLUSION</b>CLIC1 is essential for the proliferation and invasion of Hca-F cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Plasmids
/
RNA, Messenger
/
Transfection
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Gene Expression Regulation, Neoplastic
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Chloride Channels
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Carcinoma, Hepatocellular
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Reverse Transcriptase Polymerase Chain Reaction
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RNA, Small Interfering
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RNA Interference
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2010
Type:
Article