Preparation of armored RNA containing M gene of influenza H3N2 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 343-345, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-248758
ABSTRACT
<p><b>OBJECTIVE</b>To prepare the armored RNA containing M gene of influenza H3N2.</p><p><b>METHODS</b>The vector pAR-1 was constructed from expression vector pET30b in which the bacteriophage MS2 DNA fragment, containing the genes for maturase and coat protein and the pac site, was inserted. The M gene fragment of influenza A was inserted into the HindIII site downstream of the pac site on the pAR-1, which formed a new recombinant plasmid pAR-2. After the prokaryotic expression was carried out, armored RNA AR-2 containing M gene was obtained. AR-2 was purified, and then was quantified by real time RT-PCR. Moreover, the stability of AR-2 was checked.</p><p><b>RESULTS</b>AR-2 was expressed successfully. AR-2 remained stable under various storage environments. Approximately 8.9 x 10(11) copies of AR-2 particles can be purified from one milliliter of culture.</p><p><b>CONCLUSION</b>It showed that AR-2 was stable and RNase-resistant, which, as a virus surrogate, would be used as RT-PCR standards, controls and training or proficiency samples.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Reference Standards
/
RNA, Viral
/
Viral Matrix Proteins
/
Reverse Transcriptase Polymerase Chain Reaction
/
Influenza A Virus, H3N2 Subtype
/
Genetics
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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