Detection of IgM antibody against hantavirus by chemiluminescent enzyme-linked immunosorbent assay / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 171-173, 2007.
Article
in Zh
| WPRIM
| ID: wpr-248812
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a chemiluminescent enzyme-linked immunosorbent assay (CLEIA) for the detection of HTNV IgM antibody.</p><p><b>METHODS</b>Black solid 96 well microplate was coated with anti-human IgM-microantibody, HRP labeled HTNV recombinant nucleotide antigen was used as detection antigen, luminol-H2O2 was used as substrate, a CLEIA was established for the detection of HFRS patient serum IgM antibody and comparison of detection sensitivity, specificity, and stability were made between CLEIA and MacELISA.</p><p><b>RESULTS</b>Correlate coefficient of CLEIA with MacELISA is 0.97; detection sensitivity of CLEIA is 100 percent while that of MacELISA is 92.1 percent; detection specificity of CLEIA and MacELISA are both 100 percent; coefficient of variance for intra-assay and inter-assay of CLEIA are both less than 15 percent, which are comparative with MacELISA.</p><p><b>CONCLUSION</b>The established method of CLEIA is a sensitive, selective, and stable method; it is suitable for the early detection of HFRS patient serum IgM antibody.</p>
Full text:
1
Index:
WPRIM
Main subject:
Blood
/
Immunoglobulin M
/
Enzyme-Linked Immunosorbent Assay
/
Orthohantavirus
/
Allergy and Immunology
/
Hemorrhagic Fever with Renal Syndrome
/
Luminescent Measurements
/
Methods
/
Antibodies, Viral
/
Antibody Specificity
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article