Effect of hyperthermia on multidrug resistance of tongue squamous cell carcinoma cell line (Tca8113) and its multidrug resistance cell line Tca8113/CBDEA / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 447-450, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-249797
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of hyperthermia on expressions of multidrug resistance (MDR) genes in tongue squamous cell carcinoma cell line Tca8113 and its MDR cell line Tca8113/CBDEA and intracellular ADM concentration.</p><p><b>METHODS</b>Cell lines were hyperthermia-treated (42 degrees C for 0.5 h), 4 h and 24 h later real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) detected expressions of MDR relative genes (MDR1, MRPI, GST-pi) and HTS 7000 Plus Bio Assay Reader measured intracellular ADM concentration.</p><p><b>RESULTS</b>Expressions of MDR1, MRPI, GST-'pi genes in Tca8113/CBDEA significantly descended at 4 h and 24 h post-hyperthermia (P < 0.01). Expressions of MDR1, MRP1 genes in Tca8113 significantly descended at 4 h and 24 h post-hyperthermia (P < 0.05), but there was not statistical difference between 4 h and 24 h post-hyperthermia. GST-pi expression had not statistical descent at 4 h, but there was significantly descend at 24 h post-hyperthermia (P < 0.01). Drug tolerance decreased and hyperthermia increased intracellular drug concentration in Tca8113/CBDEA and Tca8113 (P < 0.01).</p><p><b>CONCLUSION</b>Hyperthermia enhance chemotherapy effect and reverse MDR genes expression in tongue squamous cell carcinoma cell line. It is suggested that hyperthermia could be used as a method to overcome MDR, which is worth more investigation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tongue Neoplasms
/
Carcinoma, Squamous Cell
/
Cell Line
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
Cell Line, Tumor
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS