Construction of recombinant fowlpox virus coexpressing gp120 of Chinese HIV-1 strain and IL-18 and its immunogenicity in mice / 生物工程学报
Chinese Journal of Biotechnology
; (12): 337-341, 2004.
Article
in Zh
| WPRIM
| ID: wpr-249986
Responsible library:
WPRO
ABSTRACT
To screening out Chinese vaccine candidate against HIV-1, Chinese vaccine strain 282E4 of fowlpox virus was used as the vector to construct the recombinant fowlpox virus (rFPV) coexpressing gp120 of Chinese HIV-1 strain and IL-18, and the recombinant virus was indentified by PCR and Western blot. The specific DNA fragment could be amplified by PCR from the genome of rFPV. Western blot analysis showed that gp120 and IL-18 could be expressed not only in chicken embryo fibroblast (CEF) cells infected by rFPV, but also in mammalian cells infected by rFPV. After the recombinant fowlpox virus was inoculated into BALB/c mice, the spleen specific CTL activities and serum antibodies in the immunized mice were detected, which demonstrated that the rFPV had good immunogenicity and could induce BALB/c mice to produce specific humoral and cellular immunity. IL-18 palyed the role of immunoadjuvant. The study lays the basis on the preparation of genetic engineering live vector vaccine against HIV-1.
Full text:
1
Index:
WPRIM
Main subject:
HIV Envelope Protein gp120
/
Adjuvants, Immunologic
/
HIV-1
/
Immunization
/
AIDS Vaccines
/
Gene Transfer Techniques
/
Vaccines, DNA
/
Interleukin-18
/
Allergy and Immunology
/
Fowlpox virus
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2004
Type:
Article