DNA-based identification of Gentiana robusta and related species / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4680-4685, 2015.
Article
in Zh
| WPRIM
| ID: wpr-250433
Responsible library:
WPRO
ABSTRACT
The alpine plant Gentiana robusta is an endemic species to the Sino-Himalayan subregion. Also, it is one of the original plants used as traditional Tibetan medicine Jie-Ji. We sequence the nuclear ribosomal internal transcribed spacer (ITS) regions, matK, rbcL, rpoC1, trnL (UAA), psbA-trnH, atpB-rbcL, trnS( GCU)-trnG(UCC), rpl20-rps12, trnL(UAA)-trnF( GAA) fragments of cp DNA in both G. robusta and such relative species as G. straminea, G. crassicaulis and G. waltonii. With Halenia elliptica as the outgroup, molecular systematic analysis reveals that G. robusta is a natural hybrid. G. straminea is the mother of hybrids, but the father is not very clear. In addition, the molecular markers for distinguishing G. robusta from the parental species or closely related species are identified, respectively. Our studies may provide valuable reference for the species identifications of medicinal plants with complex genetic backgrounds.
Full text:
1
Index:
WPRIM
Main subject:
Phylogeny
/
Plant Proteins
/
Plants, Medicinal
/
Molecular Sequence Data
/
Classification
/
DNA, Plant
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Gentiana
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DNA Barcoding, Taxonomic
/
Genetics
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Type:
Article