Environments of ethidium binding to allosteric Dna: II. Accessibility, mobility and mode of binding
Experimental & Molecular Medicine
; : 204-209, 2000.
Article
in En
| WPRIM
| ID: wpr-25122
Responsible library:
WPRO
ABSTRACT
DNA binding compounds were previously shown to bind to the right-handed DNA forms and hybrid B-Z forms in a highly cooperative manner and indicate that structural specificity plays a key role in a ligand binding to DNA. In this study, the modes of binding and structural specificity of agents to unusual DNA are examined by a variety of fluorescence techniques (intensity, polarization and quenching, etc.) to explore a reliable method to detect the association environment of ligands to deoxyoligonucleotides initially containing a B-Z junction between the left-handed Z-DNA and right-handed B-DNA. The results of fluorescence energy transfer measurement demonstrated that the ligand molecules bind to the allosterically converted DNA structures by intercalation. In the absence of high-resolution structural data, this fluorescence energy transfer measurement allowed reliable measures and infer the binding environment of ligands to the allosteric DNA structures.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Oligodeoxyribonucleotides
/
DNA
/
Circular Dichroism
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Energy Transfer
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Allosteric Regulation
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Ethidium
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Exodeoxyribonucleases
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Ligands
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Motion
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Nucleic Acid Conformation
Language:
En
Journal:
Experimental & Molecular Medicine
Year:
2000
Type:
Article