Effect of regulation of Y-box protein 1 by RNA interference on the doxorubicin induced mdr1 gene expression in K562 cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 383-387, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-251945
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of YB-1 on the transcription of induced mdr1 gene expression in K562 cells.</p><p><b>METHODS</b>K562 cells were treated with doxorubicin (DOX) at different concentrations and times. Expression of mdr1 and YB-1 genes was examined by RT-PCR and P-glycoprotein (P-gp) by flow cytometry. Cyto/nuclear protein was extracted for YB-1 detection by Western blotting. The expression of YB-1 gene in K562 cells was inhibited by YB-1 gene specific RNA interference (RNAi), then the expression of mdr1 and P-gp in YB-1 gene silenced cells treated with DOX was detected.</p><p><b>RESULTS</b>The mdr1 gene as well as its corresponding protein P-gp was highly expressed in DOX exposed K562 cells. DOX up-regulated the expression of YB-1 gene, and promoted YB-1 protein nuclear translocation. On YB-1 gene silenced, the expressions of mdr1 gene and P-gp were obviously down-regulated in DOX treated K562 cells.</p><p><b>CONCLUSION</b>Doxorubicin can induce the expression of mdr1 gene in K562 cells, which may result from the transcription of mdr1 gene by activated YB-1.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Doxorubicin
/
Gene Expression
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Neoplasm
/
ATP Binding Cassette Transporter, Subfamily B
/
K562 Cells
/
Gene Silencing
/
Protein Transport
/
RNA, Small Interfering
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2011
Type:
Article
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