Extract of Herba Siegesbeckiae on mouse rheumatoid arthritis induced by anthrogen-CIA monoclonal antibody / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 556-560, 2013.
Article
in Zh
| WPRIM
| ID: wpr-252590
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-inflammatory effect of Herba Siegesbeckiae extracts on mouse rheumatoid arthritis induced by arthrogen-CIA monoclonal antibody.</p><p><b>METHODS</b>The rheumatoid arthritis was induced by arthrogen-CIA arthritogenic monoclonal antibody in mice. The sandwich enzyme-linked immunosorbent assay was used to determine the concentration of IL-1βin mouse serum,and the content of IL-6,IL-17 and MMP-3 in supernatant of tissue homogenate of hind limb below the stifle of mice. One-way ANOVA was used for data analysis.</p><p><b>RESULTS</b>The toe swelling was attenuated in Siegesbeckiae group than that in model group [(0.218 ± 0.0307)cm(3) compared with (0.2545 ± 0.0179)cm(3), P<0.05]. The serum IL-1β level in Siegesbeckiae group was lower than that in model group [(63.74 ± 21.74)pg/ml compared with (104.96 ± 31.22)pg/ml, P<0.01]. The contents of IL-6, IL-17 and MMP-3 in tissue supernatants of Siegesbeckiae group were all lower than those of model group [(171.10 ± 48.35)pg/ml compared with (249.64 ± 75.08)pg/ml, P<0.05; (115.42 ± 56.52)pg/ml compared with (208.40 ± 88.54)pg/ml, P<0.05;(3660.31 ± 1680.99) pg/ml compared with (5420.79 ± 1201.43)pg/ml, P<0.05, respectively].</p><p><b>CONCLUSION</b>The extract of Herba Siegesbeckiae has anti-inflammatory effect on mouse rheumatoid arthritis induced by mixed arthrogen monoclonal antibody.</p>
Full text:
1
Index:
WPRIM
Main subject:
Arthritis, Experimental
/
Arthritis, Rheumatoid
/
Blood
/
Enzyme-Linked Immunosorbent Assay
/
Drugs, Chinese Herbal
/
Interleukin-6
/
Matrix Metalloproteinase 3
/
Interleukin-17
/
Therapeutic Uses
/
Drug Therapy
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2013
Type:
Article