Study on transportation of Xiaochaihu Tang in Caco-2 cell model / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1087-1090, 2011.
Article
in Zh
| WPRIM
| ID: wpr-252935
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the transportation of Xiaochaihu Tang in Caco-2 cell model.</p><p><b>METHOD</b>The safety concentration of Xiaochaihu Tang in Caco-2 cells was determined by MTT assay. Then the Caco-2 cell model was used to investigate the bi-directional transportation of Xiaochaihu Tang. The multicomponents of Xiaochaihu Tang and the influence of time were measured by high performance liquid chromatography (HPLC).</p><p><b>RESULT</b>The P(app) values of wogonoside and wogonin were (1.23 +/- 0.09) x 10(-6), (1.07 +/- 0.89) x 10(-5) cm x s(-1) from the AP side to BL side, and (2.12 +/- 0.19) x 10(-6) and (7.12 +/- 1.02) x 10(-6) cm x s(-1) from the BL side to AP side, respectively. The P(appAP --> BL)/P(app BL --> AP) ratio of wogonoside and wogonin were 0.58 and 1.49, respectively. Baicalin, baicalein and glycyrrhizic acid could not permeate the Caco-2 cell model.</p><p><b>CONCLUSION</b>The transportation of wogonoside and wogonin in Caco-2 cell model may be a passive transportation.</p>
Full text:
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Index:
WPRIM
Main subject:
Flavonoids
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Biological Transport, Active
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Drugs, Chinese Herbal
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Pharmacokinetics
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Chemistry
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Chromatography, High Pressure Liquid
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Caco-2 Cells
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Glycyrrhizic Acid
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Cell Line, Tumor
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Flavanones
Limits:
Humans
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article