Effects of Newcastle disease virus on the mitochondria of human gastric carcinoma BGC-823 cells / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 198-200, 2008.
Article
in Zh
| WPRIM
| ID: wpr-254105
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore changes in structure and function of the mitochondria of human gastric carcinoma BGC-823 cells after Newcastle disease virus (NDV) infection.</p><p><b>METHODS</b>Electron microscopy was applied to observe the structure of mitochondria; Rhodamine 123 staining was used to determine the mitochondrial membrane potential; the activity of Na(+)-K(+)-ATPase and Ca(2+)-ATPase were also determined and the release of cytochrome C was detected by Western blotting.</p><p><b>RESULTS</b>The structure of mitochondria in the tumor cells infected with NDV changed distinctly. In the infected group the activity of mitochondrial Na(+)-K(+)-ATPase and Ca(2+)-ATPase significantly declined (P < 0.01), and compared with control cells, mitochondrial trans-membrane potential was decreased. NDV infection induced the decrease of cytochrome C levels.</p><p><b>CONCLUSION</b>The effects of NDV infection on the structure and functions of mitochondria of human gastric carcinoma BGC-823 cells might play a role in the oncolysis of NDV.</p>
Full text:
1
Index:
WPRIM
Main subject:
Physiology
/
Stomach Neoplasms
/
Virology
/
Newcastle disease virus
/
Carcinoma
/
Sodium-Potassium-Exchanging ATPase
/
Cell Line, Tumor
/
Cytochromes c
/
Membrane Potential, Mitochondrial
/
Metabolism
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2008
Type:
Article