The effects and mechanisms of high glucose on the phenotype transformation of rat vascular smooth muscle cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 458-461, 2015.
Article
in Zh
| WPRIM
| ID: wpr-254990
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects and mechanisms of high glucose on the phenotype transformation of rat vascular smooth muscle cells (VSMCs).</p><p><b>METHODS</b>VSMCs ere isolated from rat thoracic aorta and the 3rd-5th VSMCs were incubated with normal glucose (5.5 mmol/L), high glucose (25 mmol/L), or high glucose (25 mmol/L) + P38 inhibitor (25 mmol/L +SB203580) for another 24 hours. Then the gene expression of osteopontin (OPN), alpha smooth-actin (alpha-SMA), matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9(MMP-9) were assayed by real time RT-PCR, the protein expression of P38 were assayed by Western blot.</p><p><b>RESULTS</b>(1) High glucose promoted the phenotype transformation of VSMCs and up-regulated the expression of MMP-2 and MMP-9. (2) High glucose promoted the phosphorylation of P38. (3) SB203580, the inhibitor of P38/MAPK signal pathway, inhibited the effects of high glucose on phenotype transformation and expression of MMP-2 and MMP-9.</p><p><b>CONCLUSION</b>High glucose may promote phenotype transformation of VSMCs via the signal pathway of P38/MAPK.</p>
Full text:
1
Index:
WPRIM
Main subject:
Aorta, Thoracic
/
Pharmacology
/
Phenotype
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Pyridines
/
Cells, Cultured
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Blotting, Western
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Actins
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9
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MAP Kinase Signaling System
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Applied Physiology
Year:
2015
Type:
Article