Neuroprotective effect of progesterone on focal cerebral ischemia/reperfusion injury in rats and its mechanism / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 231-234, 2015.
Article
in Zh
| WPRIM
| ID: wpr-255045
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the neurological protective effects of progesterone (PROG) on focal cerebral ischemia/reperfusion injury in rats and to explore its possible mechanism.</p><p><b>METHODS</b>One handred and twenty male SD rats were divided into three groups randomly: sham-operated group, middle cerebral artery occlusion ( MCAO ) group and PROG + MCAO group( n = 40). The right temporary MCAO model was established by the line-embolism method. The PROG + MCAO group rats were according to 8 mg/kg intraperitoneal injection PROG, after that 30 min, the rats were suffered ischemia/reperfusion. After rats were suffered ischemia for 2 h and reperfusion 0, 24, 48, 72 h stress, the nervous functional defect degree were evaluated by longe scoring, and the expression of two-pore domain K channel 3 (TASK3) mRNA in brain tissue were detected by the real-time PCR.</p><p><b>RESULTS</b>PROG (8 mg/kg) could significantly reduced the nervous functional defect degree in rats after ischemia/reperfusion 24, 48, 72 h (P < 0.05). The results of real-time PCR showed that the TASK3 mRNA expression in the brain tissue at all time points significantly decreased in MCAO group compared with sham-operated group (P < 0.05). However, compared with MCAO group, the expression of TASK3 mRNA in brain tissue at all time points dramatically increased in PROG + MCAO group (P < 0.05).</p><p><b>CONCLUSION</b>PROG can improve the nervous functional defect degree after focal cerebral ischemia/reperfusion injury in rats, and the mechanism might be associated with up-regulating the expression of TASK3 mRNA in brain tissue.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Pharmacology
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Progesterone
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Brain
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Reperfusion Injury
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Brain Ischemia
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Rats, Sprague-Dawley
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Neuroprotective Agents
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Infarction, Middle Cerebral Artery
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Potassium Channels, Tandem Pore Domain
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Applied Physiology
Year:
2015
Type:
Article