In vitro killing effect of adenovirus-mediated fusion gene system driven by KDR promoter on gastric cancer cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 402-405, 2006.
Article
in Zh
| WPRIM
| ID: wpr-255300
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the killing effect of adenovirus(Ad)-mediated double suicide gene driven by kinase domain-containing receptor (KDR) promoter on gastric cancer MGC-803 cells.</p><p><b>METHODS</b>The 293 packaging cells were transfected by the plasmids pAdEasy-KDR-CDglyTK to generate infectious viruses. The gastric cancer MGC-803 cells were infected by the Ad followed by treatment with 5-FC and/or ganciclovir at different concentrations. The cell-killing effects were evaluated and the bystander effects analyzed after coculture of the cells without AdKDR-CDglyTK infection with the infected cells at different ratios. The cell cycle distribution was detected by flow cytometry and the pathological changes of the cells were observed by electron microscopy.</p><p><b>RESULTS</b>The infection rate of the resultant recombinant Ad in the cells increased gradually with increment of the multiplicity of infection (MOI) of the Ads. The killing effect of CD/TK fusion gene on the MGC-803 cells was much stronger than that of either of the single suicide gene (P<0.001), and considerable bystander effect was observed. The Ad infection caused MGC-803 cell growth arrest at G(1) phase with onset of apoptotic and necrotic morphologies of the cells as seen under electron microscope.</p><p><b>CONCLUSION</b>The CD/TK fusion gene system driven by the KDR promoter possesses effective killing effect on the KDR-expressing gastric cancer MGC-803 cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Stomach Neoplasms
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Recombinant Fusion Proteins
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Genetic Therapy
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Adenoviridae
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Promoter Regions, Genetic
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Receptor Protein-Tyrosine Kinases
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Cell Line, Tumor
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Cytosine Deaminase
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Genes, Transgenic, Suicide
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article