Sensitization of human glioma SWO cell line to tumor necrosis factor-induced apoptosis by blocking phospholipase C-gamma1 signaling pathway / 南方医科大学学报
Journal of Southern Medical University
; (12): 266-269, 2006.
Article
in Zh
| WPRIM
| ID: wpr-255336
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of phospholipase C-gamma1 (PLC-gamma1) in tumor necrosis factor-alpha (TNF-alpha)-induced apoptosis of human glioma SWO cells.</p><p><b>METHODS</b>The PLC-gamma1 pathway was blocked by U73122 in SWO cells, and the inhibitory effect of TNF-alpha on SWO glioma cell proliferation with or without U73122 treatment was investigated by MTT assay. The cell apoptosis induced by TNF-alpha along or in combination with U73122 was detected by flow cytometry with PI staining. The expression of caspase-3 and Bcl-2 was detected by Western blotting.</p><p><b>RESULTS AND CONCLUSION</b>U73122 can sensitize SWO glioma cells to TNF-alpha-induced apoptosis. Blocking the PLC-gamma1 pathway may not induce apoptosis of SWO glioma cells, but can sensitize SWO glioma cells to small-dose TNF-alpha-induced apoptosis, the mechanism of which may involve down-regulation of bcl-2.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Pharmacology
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Phosphodiesterase Inhibitors
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Pyrrolidinones
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Signal Transduction
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Down-Regulation
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Cell Survival
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Blotting, Western
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Tumor Necrosis Factor-alpha
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Apoptosis
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article