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Shenfu injection attenuates neurotoxicity of bupivacaine in cultured mouse spinal cord neurons / 中华医学杂志(英文版)
Chin. med. j ; Chin. med. j;(24): 1958-1962, 2007.
Article in En | WPRIM | ID: wpr-255465
Responsible library: WPRO
ABSTRACT
<p><b>BACKGROUND</b>Our previous in vivo study in the rat demonstrates that Shenfu injection, a clinically used extract preparation from Chinese herbs, attenuates neural and cardiac toxicity induced by intravenous infusion of bupivacaine, a local anesthetic. This study was designed to investigate whether bupivacaine could induce a toxic effect in primary cultured mouse spinal cord neuron and if so, whether the Shenfu injection had a similar neuroprotective effect in the cell model.</p><p><b>METHODS</b>The spinal cords from 11- to 14-day-old fetal mice were minced and incubated. Cytarabine was added into the medium to inhibit the proliferation of non-neuronal cells. The immunocytochemical staining of beta-tubulin was used to determine the identity of cultured cells. The cultured neurons were randomly assigned into three sets treated with various doses of bupivacaine, Shenfu and bupivacaine + Shenfu, for 48 hours respectively. Cell viability in each group was analyzed by methyl thiazoleterazolium (MTT) assay.</p><p><b>RESULTS</b>The viability of the cultured neurons treated with bupivacaine at concentrations of 0.01%, 0.02%, 0.04% and 0.08% was decreased in a dose-dependent manner. Although the Shenfu injection at concentrations ranging from 1/50 to 1/12.5 (V/V) had no significant influence on the viability of cultured neurons (P < 0.05 vs control), the injection significantly increased the cellular viability of cultured neurons pretreated with 0.03% bupivacaine (P < 0.05).</p><p><b>CONCLUSION</b>Although Shenfu injection itself has no effect on spinal neurons, it was able to reduce the bupivacaine-induced neurotoxicity in vitro.</p>
Subject(s)
Full text: 1 Index: WPRIM Main subject: Spinal Cord / Bupivacaine / Drugs, Chinese Herbal / Cell Survival / Cells, Cultured / Cell Biology / Therapeutic Uses / Dose-Response Relationship, Drug / Toxicity / Injections Limits: Animals Language: En Journal: Chin. med. j Year: 2007 Type: Article
Full text: 1 Index: WPRIM Main subject: Spinal Cord / Bupivacaine / Drugs, Chinese Herbal / Cell Survival / Cells, Cultured / Cell Biology / Therapeutic Uses / Dose-Response Relationship, Drug / Toxicity / Injections Limits: Animals Language: En Journal: Chin. med. j Year: 2007 Type: Article