Crosstalk between angiotensin II and platelet derived growth factor-BB mediated signal pathways in cardiomyocytes / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 236-240, 2008.
Article
in En
| WPRIM
| ID: wpr-255729
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Angiotensin II (AngII) and platelet-derived growth factor (PDGF)-BB can induce hypertrophy in the cultured rat cardiomyocytes through different signal transduction pathways. AngII stimulates growth through G protein coupled receptor (GPCR), while PDGF-BB acts via receptor tyrosine kinase (RTK). Although there has been much development on the individual AngII and PDGF-BB mediated signal pathways, little is known about the interactions between these two factors. Therefore, the crosstalk between AngII and PDGF-BB mediated signal pathways in the rat cardiomyocytes was investigated in this study.</p><p><b>METHODS</b>Primary culture of neonatal rat ventricular myocytes was prepared. The amount of tyrosine-phosphorylated and non-phosphorylated PDGF-beta receptor, G(alphaq/11), and phospholipase C (PLC) beta(3) were measured by immunoblotting analysis. The statistical analysis was done by one-way ANOVA.</p><p><b>RESULTS</b>Tyrosine-phosphorylated PDGF-beta receptor was increased by 120.60% at 1 minute and recovered to the control level at 10 minutes after AngII stimulation. Phosphorylation of PDGF-beta receptor triggered by AngII was blocked by losartan, a specific antagonist of AT1 receptor. PLC inhibitor U73122, protein kinase C (PKC) inhibitor staurosporine (STS) and mitogen-activated ERK activating kinase (MEK) inhibitor PD98059 also inhibited the AngII-induced phosphorylation of PDGF-beta receptor. PDGF-BB slightly increased the expression of G(alphaq/11) protein.</p><p><b>CONCLUSION</b>AngII transactivates PDGF-beta receptor via AT(1) receptor-G(alphaq/11)-PLC-PKC pathway in the rat cardiomyocytes. ERK also participates in the transactivation of PDGF-beta receptor triggered by AngII.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Type C Phospholipases
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Phosphorylation
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Physiology
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Platelet-Derived Growth Factor
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Angiotensin II
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Signal Transduction
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Cells, Cultured
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Rats, Wistar
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Receptor, Platelet-Derived Growth Factor beta
Limits:
Animals
Language:
En
Journal:
Chinese Medical Journal
Year:
2008
Type:
Article