Investigation of p53 gene mutations in keloids using PCR-SSCP / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To detect gene mutations of p53 gene (exon 4-6) in fibroblasts.</p><p><b>METHODS</b>Samples of keloids were taken from 15 patients. The mutations of p53 gene were detected using polymerase chain reaction, the single-strand conformational polymorphism(SSCP) analysis and DNA sequencing.</p><p><b>RESULTS</b>Gene mutations in p53 gene exon 4, 5, and 6 were identified in all the patients with keloids.</p><p><b>CONCLUSION</b>Gene mutations resulted in keloid p53 protein losing its functions of suppressing cell processes and conducting apoptosis.</p>