Evaluation of Bronchiolar and Alveolar Cell Injuries Induced by Short- and Long-term Exposure to Sidestream Smoke
Korean Journal of Pathology
; : 151-161, 2012.
Article
in En
| WPRIM
| ID: wpr-25784
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
We investigated effects of short- and long-term exposure to sidestream smoke on the bronchiolar and alveolar cells in Sprague-Dawley rats.METHODS:
Rats were divided into five experimental groups groups 1, 2, and 3 (1-month exposure to 3, 5, and 7 cigarettes a day, respectively), groups 4 and 5 (3- and 6 month exposure to five cigarettes a day, respectively). We examined the morphologic changes, the expressions of tumor necrosis factor alpha (TNF-alpha), tumor growth factor beta1 (TGF-beta1), interlekin (IL)-1alpha, IL-1beta, Ki-67, and cytokeratin 14 and in situ apoptosis in the bronchiolar and alveolar cells on light microscopy (LM) and electron microscopic (EM) terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining.RESULTS:
LM showed the respiratory bronchiolar dilatation and alveolar wall collapse. In groups 3, 4, and 5, EM showed loss of the cilia and Clara cells with irregular size, more prominent alveolar wall collapse and dilation of alveolar duct than those of groups 1 and 2. Bronchiolar and alveolar cells showed increased expressions of TNF-alpha and TGF-beta in groups 4 and 5. LM and EM TUNEL stains showed increased apoptosis in groups 3, 4, and 5.CONCLUSIONS:
Sidestream smoke causes a bronchiolar and alveolar cell injury and the severity correlates strongly the volume and duration of exposure to sidestream smoke.Key words
Full text:
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Index:
WPRIM
Main subject:
Smoke
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Immunohistochemistry
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Transforming Growth Factor beta
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Cilia
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Tumor Necrosis Factor-alpha
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Apoptosis
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In Situ Nick-End Labeling
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Dilatation
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DNA Nucleotidylexotransferase
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Coloring Agents
Limits:
Animals
Language:
En
Journal:
Korean Journal of Pathology
Year:
2012
Type:
Article