Expression of the heat-shock protein 70 family polymorphism in A549 cell line exposed to benzo(a)pyrene / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 375-378, 2004.
Article
in Zh
| WPRIM
| ID: wpr-258737
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the pattern of polymorphism expression of heat-shock protein 70 (HSP70) family in A549 cell line treated with different concentrations of benzo(a)pyrene (BaP) and its probable biological effect.</p><p><b>METHOD</b>Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) was used for the HSP70 expression analysis.</p><p><b>RESULTS</b>2D-PAGE showed that when A549 cells were exposed to different concentrations of BaP (0.1, 1.0, 5.0, 10.0 micromol/L) for 24, 48 h respectively, the HSP72 in A549 gradually declined as BaP concentrations increased [the integral OD (IOD)] for 24 h were: 150.36 +/- 26.03, 98.57 +/- 13.34, 64.92 +/- 15.03, 34.65 +/- 19.10, 32.92 +/- 18.71 respectively, for 48 h: 126.85 +/- 17.41, 106.19 +/- 15.32, 73.64 +/- 21.02, 35.18 +/- 11.95, 16.27 +/- 9.35 respectively), while the IOD of HSP73 did not show any remarkable change (24 h: 102.29 +/- 21.24, 87.71 +/- 18.70, 71.19 +/- 14.08, 71.87 +/- 15.16, 72.78 +/- 17.31 respectively; 48 h: 86.66 +/- 16.86, 75.67 +/- 10.61, 66.83 +/- 12.63, 67.29 +/- 10.26, 91.37 +/- 13.68 respectively).</p><p><b>CONCLUSION</b>BaP can inhibit HSP72 expression and with certain dose-effect relationship, but cannot affect HSP73 expression.</p>
Full text:
1
Index:
WPRIM
Main subject:
Polymorphism, Genetic
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Benzo(a)pyrene
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HSP70 Heat-Shock Proteins
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Cell Line, Tumor
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Dose-Response Relationship, Drug
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Electrophoresis, Polyacrylamide Gel
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Genetics
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Metabolism
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2004
Type:
Article