Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus / 生物工程学报
Chinese Journal of Biotechnology
; (12): 392-396, 2003.
Article
in Zh
| WPRIM
| ID: wpr-259180
Responsible library:
WPRO
ABSTRACT
Genes encoding nucleocaspid (N) and membrane (M) protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E. coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis.
Full text:
1
Index:
WPRIM
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Viral Structural Proteins
/
Chromatography, Affinity
/
Chromatography, Ion Exchange
/
Nucleocapsid Proteins
/
Reverse Transcriptase Polymerase Chain Reaction
/
Severe acute respiratory syndrome-related coronavirus
/
Escherichia coli
/
Genetics
/
Metabolism
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2003
Type:
Article