Construction and eukaryotic expression of PVAX1-hPV58mE6E7fcGB composite gene vaccine / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 1102-1107, 2013.
Article
in Zh
| WPRIM
| ID: wpr-259759
Responsible library:
WPRO
ABSTRACT
To construct and express a composite gene vaccine for human papillomavirus 58(HPV58)-associated cervical cancer, we inserted HPV58mE6E7 fusion gene into pCI-Fc-GPI eukaryotic expression vector, constructing a recombinant plasmid named pCI-sig-HPV58mE6E7-Fc-GPI. Then we further inserted fragment of sig-HPV58mE6E7Fc-GPI into the novel vaccine vector PVAX1-IRES-GM/B7, constructing PVAX1-HPV58mE6E7FcGB composite gene vaccine. PVAX1-HPV58mE6E7FcGB vaccine was successfully constructed and identified by restriction endonuclease and sequencing analysis. Eukaryotic expression of fusion antigen sig-HPV58mE6E7-Fc-GPI and molecular ad-juvant GM-CSF and B7. 1 were proved to be realized at the same time by flow cytometry and immunofluorescence. So PVAX1-HPV58mE6E7FcGB can be taken as a candidate of therapeutic vaccine for HPV58-associated tumors and their precancerous transformations.
Full text:
1
Index:
WPRIM
Main subject:
Recombinant Proteins
/
Uterine Cervical Neoplasms
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Oncogene Proteins, Viral
/
Cancer Vaccines
/
Vaccines, DNA
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Capsid Proteins
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Allergy and Immunology
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Papillomavirus E7 Proteins
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Papillomavirus Vaccines
/
Genetics
Limits:
Female
/
Humans
Language:
Zh
Journal:
Journal of Biomedical Engineering
Year:
2013
Type:
Article