A study on the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 735-737, 2006.
Article
in Zh
| WPRIM
| ID: wpr-260612
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein.</p><p><b>METHODS</b>Four specific siRNAs were obtained by transcription in vitro. After transfection with the siRNAs into HepG2.2.15 cells for 3 days, the inhibitive effects of La protein were analyzed by Western blot; the content changes of HBsAg, HBeAg and HBV-DNA were detected by ECL and RT-PCR.</p><p><b>RESULTS</b>In comparison to normal cells, La protein was less in the cells. There was less La protein in the cells trans-infected with siRNAs. HBsAg, the HBeAg and HBV-DNA secreted by the cells transfected with siRNA were also less than that in the normal cells.</p><p><b>CONCLUSION</b>There is a correlation between La protein and HBV mRNA and the expression of HBV protein.</p>
Full text:
1
Index:
WPRIM
Main subject:
Ribonucleoproteins
/
Autoantigens
/
DNA, Viral
/
RNA, Messenger
/
RNA, Viral
/
Hepatitis B virus
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RNA, Small Interfering
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Cell Line, Tumor
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Genetics
/
Hepatitis B Surface Antigens
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2006
Type:
Article