The correlation of cyclin A gene expression with drug resistance in adult acute leukemia patients / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 243-246, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-261440
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between the expression of cyclin A and drug resistance in patients with adult acute leukemias.</p><p><b>METHODS</b>The mRNA expressions of cyclin A, mdr1, Topo II alpha and bcl-2 were measured in 64 patients with adult acute leukemia (AL) and 20 normal subjects by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>(1) The cyclin A and Topo II alpha mRNA expression levels in the treatment resistant group were significantly lower than that in the sensitive group (P < 0.01). There was no cyclin A mRNA expression in the 20 normal subjects under the same experiment condition. (2) The mdr1 and bcl-2 mRNA expression levels in the resistant group were significantly higher than that in sensitive group (P < 0.01). (3) Cyclin A and Topo II alpha gene expression levels were positively correlated (r = 0.794, P = 0.000) in the 64 AL patients, and there was a negative correlation between the gene expression levels of cyclin A and mdr1 (r = -0.337, P = 0.029) in the drug resistant group. (4) Ten AL patients with low expressions of both cyclin A and Topo II alpha were all in the resistant group. Logistic regression of binary analysis showed a significant correlation between the low expression of cyclin A and drug resistance.</p><p><b>CONCLUSION</b>Low expression of cyclin A gene might be a unfavorable prognostic factor for patients with AL and measurement of both cyclin A and Topo II alpha gene expression would predict drug resistance for AL patients.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
RNA, Messenger
/
Leukemia
/
Logistic Models
/
Acute Disease
/
DNA Topoisomerases, Type II
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
Proto-Oncogene Proteins c-bcl-2
Type of study:
Prognostic study
/
Risk factors
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2002
Type:
Article
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