Heme oxygenase-1 promotes Caco-2 cell proliferation and migration by targeting CTNND1 / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 3057-3063, 2013.
Article
in English
| WPRIM
| ID: wpr-263525
ABSTRACT
<p><b>BACKGROUND</b>Heme oxygenase-1 (HO-1) can be induced by inflammatory cytokines, oxidation, ischemia, hypoxia, and endotoxins. As a "graft survival protective gene," HO-1 is a hot spot in organ transplantation research. However, the role of HO-1 gene expression in the function of human colon adenocarcinoma cell line (Caco-2) cells has not been reported previously.</p><p><b>METHODS</b>The role of HO-1 in the proliferation and migration of Caco-2 cells was analyzed using a stable HO-1 expression plasmid. We constructed a recombinant adeno-associated virus plasmid containing the HO-1 gene, heme oxygenase 1 (HMOX1), which was transfected into Caco-2 intestinal cells. We identified a number of target genes by global microarray analysis combined with real-time polymerase chain reaction (PCR) and chromatin immunoprecipitation assay.</p><p><b>RESULTS</b>Our results showed that significant HO-1 upregulation was demonstrated in the Caco-2 cells after HO-1 transfection. Restoration of HO-1 expression promoted proliferation and invasion in vitro. The CTNND1 gene, a member of the armadillo protein family, was identified as a direct HO-1 target gene.</p><p><b>CONCLUSION</b>Overexpression of HO-1 promotes Caco-2 cell proliferation and migration by targeting the CTNND1 gene.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Gene Expression Regulation, Neoplastic
/
Cell Movement
/
Caco-2 Cells
/
Cell Proliferation
/
Heme Oxygenase-1
/
Catenins
/
Real-Time Polymerase Chain Reaction
/
Genetics
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2013
Type:
Article
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