Effect of F10 gene silencing and over-expression on cell cycle of choriocarcinoma cell line JAR and the mechanisms / 南方医科大学学报
Journal of Southern Medical University
; (12): 351-355, 2016.
Article
in Zh
| WPRIM
| ID: wpr-264042
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of F10 gene in regulating cell cycles of choriocarcinoma cells and the underlying mechanisms.</p><p><b>METHODS</b>Using untreated cells as the control, JAR cells with F10 gene silencing or stable F10 over-expression were examined for cell cycle changes by flow cytometry (FCM) and for expressions of cyclin and cyclin-dependent kinase (CDKs) with Western blotting and immunofluorescence technique.</p><p><b>RESULTS</b>JAR cells over-expressing F10 gene showed reduced duration of cell cycle compared with untreated and with cells after F10 gene silencing. In F10-over-expressing cells, Western blotting revealed significantly up-regulated expressions of cyclin A2, B1, D1, E and CDK2, 6, and 7, but not CDK4, as compared with the control cells and cells with F10 gene silencing (P<0.05), and these results were consistent with those by immunofluorescence assay.</p><p><b>CONCLUSION</b>F10 gene may accelerate cell cycle progression and promote cell proliferation by up-regulating the expressions of cyclin A2, B1, D1, E and CDK 2, 4, 6, 7 in choriocarcinoma cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Factor X
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Choriocarcinoma
/
Cell Cycle
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Cell Division
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Cyclins
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Cyclin-Dependent Kinases
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Gene Silencing
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Cell Line, Tumor
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Cell Proliferation
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Genetics
Limits:
Female
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Humans
/
Pregnancy
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2016
Type:
Article