Distribution of compact bone mesenchymal stem cells in lung tissue and bone marrow of mouse / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 171-176, 2014.
Article
in Zh
| WPRIM
| ID: wpr-264929
Responsible library:
WPRO
ABSTRACT
This study was aimed to investigate the distribution of compact bone mesenchymal stem cells(MSC) marked with lentiviral plasmid pGC FU-RFP-LV in lung tissue and bone marrow of mouse. The MSC were infected by lentivirus with infection efficiency 78%, the infected MSC were injected into BALB/c mice via tail veins in concentration of 1×10(6) /mouse. The mice were randomly divided into 4 group according to 4 time points as 1, 2, 5 and 7 days. The lung tissue and bone marrow were taken and made of frozen sections and smears respectively in order to observed the distributions of MSC. The results indicated that the lentiviral infected MSC displayed phenotypes and biological characteristics which conformed to MSC by immunophenotyping analysis and induction differentiation detection. After the MSC were infected with optimal viral titer MOI = 50, the cell growth no significantly changed; the fluorescent microscopy revealed that the distributions of MSC in bone marrow on day 1, 2, 5 and 7 were 0.50 ± 0.20, 0.67 ± 0.23, 0.53 ± 0.14, 0.33 ± 0.16; those in lung tissue were 0.55 ± 0.15, 0.47 ± 0.13, 0.29 ± 0.13, 0.26 ± 0.08. It is concluded that the distribution of MSC in lung tissue reaches a peak on day 1, while distribution of MSC in bone marrow reaches a peak on day 2. The distribution of mouse MSC relates with RFP gene expression and implantation of MSC in lung tissue and bone marrow.
Full text:
1
Index:
WPRIM
Main subject:
Bone and Bones
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Bone Marrow Cells
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Cell Biology
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Mesenchymal Stem Cells
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Lung
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Mice, Inbred BALB C
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Mice, Inbred C57BL
Limits:
Animals
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2014
Type:
Article