Effects of different extracellular matrix on differentiation of human embryonic stem cells into hematopoietic progenitor cells / 中国实验血液学杂志

ABSTRACT

This study was purposed to establish a new inducing system for differentiation of human embryonic stem cells into hematopoietic progenitor cells and to explore the effect of different extracellular matrices (DEM) on production of hematopoietic cells. The 3 kinds of extracellular matrices-matrigel, fibronectin and IV type collagen (collagen IV) were chosen to package cultured plates, the direct adherent culture on extracellular matrix was used, and the hematopoietic growth factors were added into cultured plates to induce the differentiation of human embryonic stem cells into hematopoietic progenitor cells. The hematopoietic colony forming unit assay was used to determine the yielded colony forming cells, the flow cytometry and real-time quantitative PCR were used to detect the expression of markers specific to hematopoiesis and the effect of 3 extracellular matrices on production of hematopoietic progenitor cells was compared. The results showed that after being induced for 14 days, the total yield of colony forming cells, the ratio of CD34(+) cells and the expression level of SCL and CD34 on collagen IV were significantly higher than those on matrigel and fibronectin groups (P < 0.05). It is concluded that human embryonic stem cells can efficiently differentiate into hematopoietic progenitor cells by direct adherent culture on extracellular matrices, and the collagen IV can improve the hematopoietic differentiation of human embryonic stem cells.