Expression of RASSF1A and RASSF1C transcripts in human primary ovarian cancers / 中华病理学杂志
Chinese Journal of Pathology
; (12): 150-153, 2005.
Article
in Zh
| WPRIM
| ID: wpr-265167
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of two major alternative transcripts of RASSF1 gene (RASSF1A and RASSF1C) in human primary ovarian cancers and their biological implication as a new tumor suppressor gene.</p><p><b>METHODS</b>Reverse transcription-polymerase chain reaction (RT-PCR) and laser capture microdissection (LCM) were used to determine mRNA expression of the two major alternative transcripts of RASSF1 gene (RASSF1A and RASSF1C) in 3 ovarian cancer cell lines and 80 cases of primary ovarian cancers.</p><p><b>RESULTS</b>RASSF1A mRNA was undetectable in SK-OV-3 cell line. Expression of RASSF1A and RASSF1C in 80 primary ovarian cancers were 40.0% (32/80) and 91.3% (73/80) respectively. RASSF1A mRNA expression was detectable more frequently in stage I and II (71.4%, 10/14; 75.0%, 9/12) than in stage III and IV ovarian cancers (26.7%, 12/45; 14.1%, 1/9) (P < 0.05). The expression level was also higher in well and moderately differentiated tumor groups (58.6%, 17/29; 50.0%, 10/20) than in poorly differentiated tumor group (16.1%, 5/31) (P < 0.05).</p><p><b>CONCLUSION</b>There is a preferential loss of RASSF1A expression in human ovarian cancers and its expression is correlated with the tumor stage and the degree of histological differentiation which, as a tumor suppressor gene, might play an important role in the tumorigenesis of human primary ovarian cancer.</p>
Full text:
1
Index:
WPRIM
Main subject:
Ovarian Neoplasms
/
Pathology
/
Prognosis
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RNA, Messenger
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Gene Expression Regulation, Neoplastic
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Genes, Tumor Suppressor
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Cystadenocarcinoma, Serous
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Cystadenocarcinoma, Mucinous
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Carcinoma, Endometrioid
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Reverse Transcriptase Polymerase Chain Reaction
Type of study:
Prognostic_studies
Limits:
Female
/
Humans
Language:
Zh
Journal:
Chinese Journal of Pathology
Year:
2005
Type:
Article