Expressions of cysteine-rich secretory protein 2 in asthenospermia / 中华男科学杂志
National Journal of Andrology
;
(12): 203-207, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-266190
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mRNA and protein expression levels of cysteine-rich secretory protein 2 (CRISP2) in the sperm of asthenospermia patients, and explore their relationship with sperm motility and related molecular mechanism.</p><p><b>METHODS</b>We collected 78 semen samples from adult male patients with asthenospermia and another 70 from healthy volunteers as controls. We extracted total RNA and total protein from the sperm following purification of the sperm by Percoll gradient centrifugation, and detected the relative expressions of CRISP2 mRNA and protein in the two groups by RT-PCR, SYBR Green real-time PCR and Western blot.</p><p><b>RESULTS</b>The expression of CRISP2 mRNA was down-regulated by 4.3 times and that of the CRISP2 protein by 1.71 times in the asthenospermia patients, significantly lower than in the normal control group (P < 0.05).</p><p><b>CONCLUSION</b>The down-regulation of CRISP2 mRNA and protein expressions in the sperm of asthenospermia patients may be closely related with decreased sperm motility, which suggests that CRISP2 may serve as a potential molecular target for the research of asthenospermia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Sperm Motility
/
Spermatozoa
/
Glycoproteins
/
Case-Control Studies
/
Asthenozoospermia
/
Genetics
/
Metabolism
Type of study:
Observational study
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2011
Type:
Article
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