mRNA expression alteration of two-pore potassium channels in the brain of beta-amyloid peptide25-35-induced memory impaired rats / 药学学报
Acta Pharmaceutica Sinica
; (12): 721-724, 2003.
Article
in Zh
| WPRIM
| ID: wpr-266618
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To study mRNA expression alteration of two-pore potassium channels in the brain of beta-amyloid peptide25-35 (beta-AP25-35)-induced memory impaired rats.</p><p><b>METHODS</b>Memory impairments induced in rats by single icv injection of beta-AP25-35 (2 mmol.L-1) 5 microL were assessed in the Morris water maze test. The mRNA expression levels of three two-pore potassium channels TREK-1, TREK-2 and TRAAK were detected in rat cerebral cortex and hippocampus by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>In the Morris water maze test, the escape latencies of the beta-AP25-35-treated rats were longer than those of the control group in 1st, 2nd and 4th training day, suggesting that the memory of beta-AP25-35-treated rats was obviously impaired. Compared with the control group, the mRNA levels of TREK-1, TREK-2 and TRAAK in the hippocampus of the beta-AP25-35-treated rats were increased by 40.0%, 27.9% and 18.9%, respectively; while no significant change of TREK-1, TREK-2 and TRAAK mRNA levels was observed in the cortex.</p><p><b>CONCLUSION</b>The mRNA expression levels of two-pore potassium channels were increased significantly in the brain of beta-AP25-35-induced memory impaired rats.</p>
Full text:
1
Index:
WPRIM
Main subject:
Peptide Fragments
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RNA, Messenger
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Potassium Channels
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Cerebral Cortex
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Amyloid beta-Peptides
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Rats, Sprague-Dawley
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Maze Learning
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Reverse Transcriptase Polymerase Chain Reaction
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Potassium Channels, Tandem Pore Domain
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Genetics
Limits:
Animals
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2003
Type:
Article