Inhibition of dexamethasone, indomethacin and resveratrol on matrix metalloproteinase-9 and the mechanism of inhibition / 药学学报
Acta Pharmaceutica Sinica
; (12): 501-504, 2003.
Article
in Zh
| WPRIM
| ID: wpr-266651
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate the expression of matrix metalloproteinase-9 (MMP-9) in mouse ears induced with croton oil and the inhibitory effect of dexamethasone, indomethacin and resveratrol on MMP-9 expression, and further explore the relationship between anti-inflammation and MMP-9 inhibition of these three medicines.</p><p><b>METHODS</b>Immuno-histochemistry was used to detect the expression of MMP-9 in mouse ears. Expression of MMP-9 in U937 cells was analyzed by gelatin zymography.</p><p><b>RESULTS</b>Mouse ear edema induced with croton oil was inhibited significantly by dexamethasone and indomethacin at the dose of 10 mg.kg-1 and resveratrol at 50 mg.kg-1 administered subcutaneously. The inhibitory rate was 76.2% (P < 0.001), 56.7% (P < 0.001) and 36.9% (P < 0.001) respectively. The MMP-9 expression increased in mouse ears induced with croton oil and inhibited by dexamethasone, indomethacin and resveratrol at above doses. Gelatin zymography results showed that MMP-9 expression in U937 cells increased significantly after exposed to PMA at 1 x 10(-8) mol.L-1 (P < 0.001); MMP-9 expression induced with phorbol myristate acetate(PMA) was inhibited by dexamethasone at 1 x 10(-9), 1 x 10(-7) and 1 x 10(-5) mol.L-1, indomethacin at 1 x 10(-6) and 1 x 10(-5) mol.L-1 and resveratrol at 1 x 10(-6) and 1 x 10(-5) mol.L-1.</p><p><b>CONCLUSION</b>The inhibition of MMP-9 expression may be one of the anti-inflammatory mechanisms of dexamethasone, indomethacin and resveratrol.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Stilbenes
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Croton Oil
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Dexamethasone
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Random Allocation
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Anti-Inflammatory Agents, Non-Steroidal
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Indomethacin
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U937 Cells
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Matrix Metalloproteinase 9
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Ear Diseases
Type of study:
Clinical_trials
Limits:
Animals
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Humans
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Male
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2003
Type:
Article