Adipose tissue sample preparation for scanning electron microscopy / 南方医科大学学报
Journal of Southern Medical University
; (12): 435-436, 2012.
Article
in Zh
| WPRIM
| ID: wpr-267581
Responsible library:
WPRO
ABSTRACT
Conventional sample preparation technique for adipose tissue is difficult to achieve satisfactory results for scanning electron microscopy (SEM). We adopted a strategy of postfix with osmium tetroxide to stabilize the fatty acids, phospholipids protein, and hence the membrane structure. Also by extending the dehydration time to fully replace the organic solvents, we achieved satisfactory results for SEM of adipose samples.
Full text:
1
Index:
WPRIM
Main subject:
Specimen Handling
/
Microscopy, Electron, Scanning
/
Adipose Tissue
/
Rats, Sprague-Dawley
/
Methods
Limits:
Animals
/
Female
/
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article