Differential expression of hsa-miR-126 and hsa-miR-518b in esophageal squamous carcinoma / 南方医科大学学报
Journal of Southern Medical University
; (12): 23-27, 2011.
Article
in Zh
| WPRIM
| ID: wpr-267680
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify the differentially expressed microRNAs (miRNAs) between esophageal squamous carcinoma (ESC) and adjacent non-tumorous tissue (NT).</p><p><b>METHODS</b>The expression levels of the miRNAs were detected in 3 fresh ESC and NT samples by hybridization with miRNAs microarray chip. Real-time quantitative RT-PCR was performed to confirm the results of the microarray analysis. The expressions of hsa-miR-126 and hsa-miR-518b in ESC were validated by real-time quantitative RT-PCR in another independent 15 matched samples.</p><p><b>RESULTS</b>A total of 11 miRNAs exhibited differential expressions in ESC samples as compared to their expressions in the NT samples, including a 1 up-regulated miRNA and 10 down-regulated miRNAs. Compared with normal esophageal samples, the ESC tissues showed up-regulated hsa-miR-126 and down-regulated hsa-miR-518b expression.</p><p><b>CONCLUSION</b>hsa-miR-126 and hsa-miR-518b are differentially expressed in ESC, and they might play important roles in the carcinogenesis and progression of ESC.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Esophageal Neoplasms
/
Tumor Cells, Cultured
/
Carcinoma, Squamous Cell
/
Biomarkers, Tumor
/
Gene Expression Regulation, Neoplastic
/
Gene Expression Profiling
/
MicroRNAs
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article