Toxic effects of CD-TK double suicidal gene system against prostate carcinoma cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 479-481, 2007.
Article
in Zh
| WPRIM
| ID: wpr-268099
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the toxic effects of the CD-TK fusion gene systems against prostate carcinoma cell line RM-1 for assessing the value of suicidal gene therapy for prostate carcinoma.</p><p><b>METHODS</b>CD-TK fusion gene and green fluorescent protein (GFP) gene were transfected into RM-1 cells through adenovirus vectors. RT-PCR was used to demonstrate successful transfection and transcription of the suicidal genes. The toxic effects of 5-FC and GCV used alone or in combination on the transfected cells were observed by MTT assay, with the non-transfected RM-1 cells serving as control.</p><p><b>RESULTS</b>Cytotoxic activity of CD/5-FC and TK/GCV systems against RM-1 cells was observed, and combined treatment with the two drugs resulted in significantly lowered survival of CD-TK-expressing cells (P<0.05). After exposure to 5-FC and GCV for 72 h, the survival rate of the transfected cells decreased to 71.56% and 47.27%, respectively, and their combined use resulted in a survival rate as low as 18.46%.</p><p><b>CONCLUSION</b>CD-TK fusion double suicidal gene system can produce significantly stronger toxic effect against RM-1 cells in vitro than either of suicidal genes.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Prostatic Neoplasms
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Therapeutics
/
Thymidine Kinase
/
Transfection
/
Genetic Therapy
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Reverse Transcriptase Polymerase Chain Reaction
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Cell Line, Tumor
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Cytosine Deaminase
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Genes, Transgenic, Suicide
Limits:
Humans
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Male
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article