The cellular and molecular mechanism of inhibitory effect of asiaticoside on capsular contracture following breast augmentation / 中华整形外科杂志
Chinese Journal of Plastic Surgery
; (6): 44-47, 2011.
Article
in Zh
| WPRIM
| ID: wpr-268647
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the cellular and molecular mechanism of the inhibitory effect of asiaticoside on capsular contracture following breast augmentation.</p><p><b>METHODS</b>Contracture capsule derived fibroblasts were cultured in medium with different concentration of asiaticoside. The cell proliferation, collage synthesis and alpha-SMA expression were detected by means of 3H-thymidine incorporation, 3H-proline incorporation, and Western-blot. The results were analyzed by SPSS 11.0 with t test.</p><p><b>RESULTS</b>DNA and collagen synthesis of fibroblasts were dramatically inhibited when the asiaticoside reached the concentration of 50 mg/L. The inhibitory rate was 34.7% and 30.1% respectively, showing a significant difference from that in control group (P < 0.05). The inhibitory effect increased with the rise of the asiaticoside concentration in a dose-dependent manner. When the concentration of asiaticoside reached 25 mg/L, the expression of alpha-SMA was down-regulated with an activation index of 1.673, showing a significant difference when compared with that in control group (P < 0.05).</p><p><b>CONCLUSIONS</b>asiaticoside can effectively inhibit the DNA and collagen synthesis of capsule-derived fibroblasts. The trans-differentiation of fibroblast to myo-fibroblasts is also prevented by it.</p>
Full text:
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Index:
WPRIM
Main subject:
Postoperative Complications
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General Surgery
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Triterpenes
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Breast
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DNA
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Cells, Cultured
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Collagen
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Mammaplasty
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Contracture
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Cell Biology
Limits:
Adult
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Female
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Humans
Language:
Zh
Journal:
Chinese Journal of Plastic Surgery
Year:
2011
Type:
Article