The study on the role of the soluble factors secreted by engineered cartilage in inducing bone marrow stromal cells chondrogenesis / 中华整形外科杂志
Chinese Journal of Plastic Surgery
; (6): 215-220, 2010.
Article
in Zh
| WPRIM
| ID: wpr-268702
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of the soluble factors secreted by tissue engineered cartilage in promoting bone marrow stromal cells (BMSCs) chondrogenesis as an important aspect.</p><p><b>METHODS</b>Porcine BMSCs, chondrocytes and dermal fibroblasts were respectively in vitro expanded and then seeded onto the polyglycolic acid/polylactic acid (PGA/PLA) scaffold. After 3 days, they were indirectly co-cultured by transwell. BMSCs-scaffold constructs were co-cultured with chondrocytes-scaffold constructs as experiment group (Exp), while co-cultured with fibroblasts-scaffold constructs as control group. BMSCs with the same cell number were seeded onto the scaffolds as another control group. There were 3 specimens in each group. All specimens were harvested after in vitro indirect co-culture for 8 weeks. Gross observation, histology, immunohistochemistry and RT-PCR were used to evaluate the results.</p><p><b>RESULTS</b>The BMSCs-scaffold constructs co-cultured with chondrocytes-scaffold shrunk gradually during in vitro culture, but formed the mature lacuna structures and metachromatic matrices, collagen II expression could be observed by immunohistochemistry and RT-PCR examination. In the control group, the constructs shrunk greatly during in vitro culture and showed mainly fibrous tissue.</p><p><b>CONCLUSIONS</b>The soluble factors secreted by chondrocytes can solely induce chondrogenic differentiation of BMSCs and thus promote the in vitro chondrogenesis of BMSCs.</p>
Full text:
1
Index:
WPRIM
Main subject:
Swine
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Bone Marrow Cells
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Cell Differentiation
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Cells, Cultured
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Stromal Cells
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Coculture Techniques
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Chondrocytes
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Bodily Secretions
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Chondrogenesis
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Cell Biology
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Plastic Surgery
Year:
2010
Type:
Article