Effect of androsterone on scavenger receptor class B type I in human vascular endothelial cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1344-1347, 2009.
Article
in Zh
| WPRIM
| ID: wpr-268764
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of FXR on scavenger receptor class B type I (SR-BI) expression.</p><p><b>METHODS</b>Human vascular endothelium Eahy926 cells were treated with FXR agonist androsterone, and the specific target gene of FXR SHP mRNA was detected by RT-PCR. SR-BI mRNA and protein were determined using RT-PCR, real-time PCR and Western blotting.</p><p><b>RESULTS</b>The level of SHP mRNA in Eahy926 cells increased after androsterone treatment at different concentrations for 24 h, demonstrating FXR activation in the cells. RT-PCR, real-time PCR and Western blotting detected increased SR-BI expression at both mRNA and protein levels after FXR activation.</p><p><b>CONCLUSION</b>FXR increases the expression of SR-BI in human vascular endothelium cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Cell Line
/
Receptors, Cytoplasmic and Nuclear
/
Endothelial Cells
/
Scavenger Receptors, Class B
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Androsterone
/
Metabolism
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article