A probable novel splicing isoform of human vascular endothelial growth factor / 南方医科大学学报
Journal of Southern Medical University
;
(12): 755-759, 2012.
Article
in English
| WPRIM
| ID: wpr-269003
ABSTRACT
<p><b>OBJECTIVE</b>To characterize a new alternative splicing isoform of human vascular endothelial growth factor (VEGF) gene.</p><p><b>METHODS</b>The total RNA was extracted from the lung tissue of a legally aborted 4-month-old fetus and amplified by RT-PCR. The amplified product was cloned into the plasmid pMD18-T and plasmid pcDNA3.1- for sequence analysis.</p><p><b>RESULTS</b>Electrophoresis of the RT-PCR products displayed one short band for VEGF(121) (487 bp) and a long band. The latter was characterized to contain two fragments one was normal VEGF(165) (619 bp), and the other (639 bp) had an identical nucleotide sequence to VEGF(165) with a 20 bp fragment inserted between exons 3 and 4. Sequence analysis showed that this 20-bp nucleotide was inserted from the 3' end of the third intron containing a splicing signal, thus causing shift mutation in the reading frame of VEGF gene and early appearance of the stop codon UAG in the middle of exon 4.</p><p><b>CONCLUSION</b>A new alternative splicing isoform of VEGF probably exists in the lung tissue of a legally aborted human fetus, and its biological significance remains to be further investigated.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Gene Expression
/
Exons
/
Frameshift Mutation
/
Amino Acid Sequence
/
Classification
/
Alternative Splicing
/
Protein Isoforms
/
Reverse Transcriptase Polymerase Chain Reaction
/
Vascular Endothelial Growth Factor A
/
Genetics
Limits:
Humans
Language:
English
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
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