Effect of adenoviral vectors on the differentiation potential of human bone marrow mesenchymal stem cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1132-1135, 2008.
Article
in Zh
| WPRIM
| ID: wpr-270194
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of adenoviral vector infection on the differentiation potential of human bone marrow mesenchymal stem cells (hMSCs).</p><p><b>METHODS</b>The third-passage hMSCs were infected with the recombinant adenovirus expressing green fluorescence protein (GFP) for 2, 4, 8 and 16 days. RT-PCR was used to detect the mRNA expression of endodermal marker CYP 51, mesodermal marker SM22alpha, ectodermal marker nestin, pluripotent marker oct-4 and the alternative splicing factor nPTB. Seven days after adenovirus infection, the hMSCs were cultured in the presence of adipogenic agents for 14 days, and the adipose cells differentiated from hMSCs were detected with oil red O staining.</p><p><b>RESULTS</b>Compared with normal hMSCs, the cell infected with the adenovirus for 2, 4, 8 and 16 days showed no obvious down-regulation of CYP51, SM22alpha, nestin, OCT4 or nPTB. The hMSCs 7 days after adenovirus infection were induced to differentiate into adipose cells, with a similar differentiation rate to that of normal hMSCs. CONCLUSION The differentiation potential of hMSCs is not affected by adenovirus infection, suggesting that adenovirus can be used as the gene delivery vector in MSC differentiation studies.</p>
Full text:
1
Index:
WPRIM
Main subject:
Physiology
/
RNA, Messenger
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Bone Marrow Cells
/
Cell Differentiation
/
Cells, Cultured
/
Adenoviridae
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Reverse Transcriptase Polymerase Chain Reaction
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Cell Biology
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Green Fluorescent Proteins
/
Host-Pathogen Interactions
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article