Effects of lead exposure on expression of mGluR5 in mRNA and protein levels of the cultured hippocampal neurons / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 266-270, 2007.
Article
in Zh
| WPRIM
| ID: wpr-270507
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects on the expression of mGluR5 mRNA and protein levels in primarily cultured hippocampal neurons after lead exposure.</p><p><b>METHODS</b>Primary embryonic rat hippocampal neuronal culture was prepared. On the 3(rd) day of incubation, lead chloride solution was added into medium to produce four different lead exposure levels: 0, 1 x 10(-8) mol/L, 1 x 10(-6) mol/L, 1 x 10(-4) mol/L Pb(2+). After 10 days of incubation, the neurons were collected to measure the alteration of mGluR5 mRNA expression by real-time fluorescent quantity PCR and the expression of mGluR5 in protein level by Western blot.</p><p><b>RESULTS</b>The studies revealed that mGluR5 mRNA expression was down-regulated after lead exposure in a dose-dependent manner. The mGluR5 mRNA expression of the lower lead-exposed neurons (Pb(2+) 10(-8) mol/L), the medium lead-exposed neurons(Pb(2+) 10(-6) mol/L), the higher lead-exposed neurons(Pb(2+) 10(-4) mol/L) were 0.724, 0.421, 0.321 times less than that of the controls, respectively. The Western blot demonstrated that mGluR5 expression in protein level should be decreased after lead exposure.</p><p><b>CONCLUSION</b>The expression of mGluR5 in mRNA and protein levels should be down-regulated after lead exposure at different lead levels in a dose-dependent manner.</p>
Full text:
1
Index:
WPRIM
Main subject:
RNA, Messenger
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Cells, Cultured
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Rats, Sprague-Dawley
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Receptors, Metabotropic Glutamate
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Lead Poisoning, Nervous System
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Toxicity
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Receptor, Metabotropic Glutamate 5
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Genetics
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Hippocampus
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Lead
Limits:
Animals
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Pregnancy
Language:
Zh
Journal:
Chinese Journal of Preventive Medicine
Year:
2007
Type:
Article