Crystalline nickel sulfide-induced genomic instability in transformed human broncho-epithelial cells / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 57-59, 2004.
Article
in Zh
| WPRIM
| ID: wpr-272042
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the genomic instability in the 16 human broncho-epithelial (16HBE) cells induced by crystalline nickel sulfide so as to provide the scientific basis for further study of nickel-induced cancer molecular mechanism.</p><p><b>METHODS</b>To analyse the genomic instability in transformed 16HBE cells induced by crystalline nickel sulfide by random amplified polymorphic DNA (RAPD).</p><p><b>RESULTS</b>All the 7 random primers selected could amplify 1 - 6 clear PCR bands. There were no significant differences between transformed 16HBE cells and negative control cells in the 4th, 5th, and 7th primers, but in the rest 4 primers there were significant differences, with special PCR bands for the same primer, indicating that genomic instability in transformed 16 HBE cells was induced by crystalline nickel sulfide.</p><p><b>CONCLUSION</b>Crystalline nickel sulfide could induce genomic instability in 16HBE cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
DNA
/
Cell Line, Transformed
/
Random Amplified Polymorphic DNA Technique
/
Crystallization
/
Genomic Instability
/
Epithelial Cells
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Toxicity
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Genetics
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Metabolism
/
Nickel
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2004
Type:
Article