Expression of IL-27 in multiple myeloma and its cell lines / 中华血液学杂志
Chinese Journal of Hematology
; (12): 626-628, 2013.
Article
in Zh
| WPRIM
| ID: wpr-272151
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression and significance of IL-27 in patients with multiple myeloma (MM) and in the supernatant of MM cell lines U266 and RPMI8226 cells culture medium.</p><p><b>METHODS</b>A total of 20 MM patients and 20 controls were enrolled in this study. The expressions of IL-27 and IL-6 in MM patient's blood plasma, and the expression of IL-27 in U266 and RPMI8226 culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of IL-27 in mononuclear cells of MM patients' peripheral blood was measured by real-time quantitative polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The levels of IL-27 in plasma of MM patients and normal controls were (61.82 ± 8.01) ng/L and (8.29 ± 4.41) ng/L (P<0.05), and those of IL-6 were (45.62 ± 1.24) ng/L and (2.27 ± 0.18) ng/L (P<0.05), respectively. The levels of IL-27 in U266 and RPMI8226 culture supernatant were (50.06 ± 5.72) ng/L and (335.47 ± 41.88) ng/L. RT-PCR revealed that the levels of IL-27 mRNA were up-regulated in MM patients compared to controls.</p><p><b>CONCLUSION</b>High expression of IL-27 is observed in MM patients and MM cell lines U266 and RPMI8226 cells. IL-27 may play a important role in pathogenesis of MM.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Blood
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Case-Control Studies
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Interleukins
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Interleukin-6
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Cell Line, Tumor
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Metabolism
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Multiple Myeloma
Type of study:
Observational_studies
Limits:
Adult
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Aged
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2013
Type:
Article