Therapeutic study of leukemia by pegylated liposomal daunorubicin / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 746-750, 2014.
Article
in Zh
| WPRIM
| ID: wpr-272299
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the antitumor effect and toxicity of pegylated liposomal daunorubicin (PL-DNR) on leukemia.</p><p><b>METHODS</b>PL-DNR was prepared by dry lipid hydration and remote loading, and its physicochemical indexes were analyzed. The inhibiting effect of PL-DNR on leukemia cells was observed in terms of in vitro cytotoxicity experiment. The therapeutic effect in vivo was assessed by tumor inhibition in leukemia L1210-bearing mice. Apoptosis in cardiomyocytes was detected using the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling method (TUNEL staining).</p><p><b>RESULTS</b>The average diameter of PL-DNR was (110 ± 10)nm and the encapsulation efficiency was 94.21%. The in vitro cytotoxicity experiment showed that the inhibiting ability of PL-DNR in the treatment groups was continuously enhanced as the experiment proceeded. The in vivo pharmacodynamic experiment also indicated obvious tumor-inhibiting effect of PL-DNR. At the end of the experiment, the tumor volume of the PL-DNR group was (433.71 ± 234.77)mm(3), significantly smaller than that of (1 293.77 ± 381.26) mm(3) in the DNR group (P < 0.05). Moreover, the tumor weight of the PL-DNR group was (0.66 ± 0.29)g and that of the DNR group was (1.25 ± 0.43)g (P < 0.05). The myocardial toxicity experiment showed that the median apoptosis index of cardiomyocytes in the PL-DNR group was 13.83%, significantly lower than that of 42.67% in the DNR group (P < 0.05), indicating a lower toxicity of PL-DNR to the myocardium.</p><p><b>CONCLUSION</b>Compared with the free DNR, PL-DNR can improve the therapeutic effect on leukemia and reduce the.</p>
Full text:
1
Index:
WPRIM
Main subject:
Therapeutics
/
Leukemia
/
Daunorubicin
/
Apoptosis
/
Therapeutic Uses
/
Antibiotics, Antineoplastic
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Oncology
Year:
2014
Type:
Article